Journal: CNS Neuroscience & Therapeutics

Article Title: Microbial lipopolysaccharide‐induced inflammation contributes to cognitive impairment and white matter lesion progression in diet‐induced obese mice with chronic cerebral hypoperfusion

doi: 10.1111/cns.14301

Figure Lengend Snippet: High‐fat diet feeding increases lipopolysaccharide (LPS) concentration, inflammatory cytokine concentrations, TLR4 expression, and blood–brain barrier (BBB) permeability in the corpus callosum of wild‐type mice 14 and 28 days after bilateral carotid artery stenosis. (A) Western blotting and densitometric analyses of LPS and TLR4 ( n = 6 per group). (B) IL‐6 ( n = 5 per group) and IL‐1β levels ( n = 6 per group). (C) Western blotting and densitometric analysis of occludin ( n = 6 per group). (D) BBB permeability as assessed by IgG ( n = 6 per group). Scale bar: 50 μm. Results are presented as mean ± SD. * p < 0.05, ** p < 0.01.

Article Snippet: The membranes were blocked in Block Ace (Dainichi‐Seiyaku) and then incubated overnight at 4°C with antibodies against myelin basic protein (MBP; 1:5000; Abcam), LPS (1:5000; Abcam), TLR4 (1:5000; Santa Cruz Biotechnology), occludin (1:5000; Abcam), or α‐tubulin (1:10000; Santa Cruz Biotechnology), followed by incubation with peroxidase‐conjugated secondary antibodies (1:5000; Santa Cruz Biotechnology).

Techniques: Concentration Assay, Expressing, Permeability, Western Blot